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However that may be, successive hydrolyses of this molecule must furnish derivatives of decreasing molecular weight. The first derivatives, still very complex, will have properties similar to those of the original material, while their structure will remain practically the same. These are the albumoses. Then we have the peptones, or the natural polypeptides. Their molecular weight will be about 2400 for the first products and about 400 for the second. The average molecular weight will continue to decrease progressively, to finally reach a value between 75, corresponding to glycin, and 240, which represents cystin, amino-adds which appear in the extreme decomposition of albuminoids.
Of animal, it is found that five to ten minutes after the introduction of the solution, the blood which is collected is no longer spontaneously coagulable. This can then be centrifuged and a peptonized plasma obtained. It should be noted, in passing, that these different plasmas, not spontaneously coagulable, are, however, rich in fibrinogen, and that they can serve, especially the goose plasma and the oxalated plasma, as reagents to show the presence of thrombin in a liquid. Arthus has even shown that fluoride plasma can be utilized as a quantitative reagent for this enzyme.
1. First of all there is observed a progressive diminution in the average molecular weight of the substance undergoing hydrolysis. We have stated that albuminoid substances appear to be compounds of very high molecular weight; Schutzenberger has attributed to albumin the value of 5691; Sabonijeff, by applying the cryoscopic method to various solutions of albuminoids, found 15,000. However that may be, successive hydrolyses of this molecule must furnish derivatives of decreasing molecular weight.